Production of Lily Symptomless Virus-free Plants by Shoot Meristem Tip Culture and In Vitro Thermotherapy

elimination of bean yellow mosaic virus through thermotherapy combined with meristem-tip culture in gladiolus corms

bean yellow mosaic virus (bymv, potyvirus, potyviridae) causes serious disease in gladiolus spp. in this work, the possibility of obtaining bymv free plant material from virus infected gladiolus corms was studied. thermotherapy, meristem-tip culture and combination of both techniques on infected corms/meristem-tip explants (0.5–1 mm in length) resulted in bymv elimination up to 15.38, 78.04 and...

Thermotherapy, chemotherapy, and meristem culture in banana.

Bananas that provide a staple food to the millions of people are adversely affected by several viruses such as Banana bunchy Top Virus (BBTV), Banana Streak Virus (BSV), and Cucumber Mosaic Virus (CMV). These viruses are known to have a devastating effect on crop production and constraint to the international exchange and conservation of banana germplasm-a cornerstone for breeding new cultivars...

Meristem-tip culture for propagation and virus elimination.

elimination of potyviruses from two commercial iranian garlic (allium sativum l.) populations through thermotherapy and by meristem tip culture

elimination of potyviruses from two commercial iranian garlic populations, hamedan and azar-shahr, through thermotherapy and by meristem tip culture were investigated. the infected bulbs of either one of the populations were initially treated at 30o c for one week and then at 36oc for 5 weeks. the meristems of cloves at two sizes of 0.4-0.6 mm and 0.6-0.8 mm were then cultured on ms medium supp...

Production of Indian Citrus Ringspot Virus Free Plants of Kinnow Employing Chemotherapy Coupled with Shoot Tip Grafting

Indian citrus ringspot virus (ICRSV) is known to cause serious problem in Kinnow (Citrus nobilis Lour × C. deliciosa Tenora). This paper reports the elimination of ICRSV from Kinnow by chemotherapy coupled with shoot tip grafting under in vitro conditions. Nodal segments from infected mother plant (indexed by indirect ELISA and RT-PCR) were cultured on MS medium containing 2-iP (1mg/l) and malt...